Sanger sequencing reaction mixtures included purified template DNA, a primer, DNA polymerase, and all four dNTPs. Each reaction also included a radiolabeled ddNTP version of just one of the four nucleotides. Only a small amount of each ddNTP was added, however, to ensure that a fraction of the total DNA fragments produced stopped at each occurrence of that base. As with previous methods, separating fragments via length and performing autoradiography allowed scientists to read the final sequence.
[&:first-child]:overflow-hidden [&:first-child]:max-h-full"。关于这个话题，safew官方版本下载提供了深入分析
，这一点在爱思助手下载最新版本中也有详细论述
what about the other linear lookaround approaches?，这一点在safew官方版本下载中也有详细论述
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